IHC video webinar series

 

Session 1

Session 2

Session 3

Session 4

Session 5

 

Session 1: Essentials of Immunohistochemistry

Session 2: Selecting Your Reagents - Not All Reagents are Created Equal

Session 3: Selecting Your Ancillary Reagents - Sometimes the Little Things Make All the Difference

Session 4: Optimizing Your Protocol - Maximizing the Performance of Your Reagents

Session 5: Beyond the Basics - Advanced Detection Protocols

 

 

 

 

 

 

Welcome! If you're not already enrolled in this course, sign up here to get the rest of the sessions emailed to you for free.

Welcome to Session 1 of Considerations For Designing Immunohistochemical Procedures. In this session we’re going to cover the essentials of immunohistochemistry.

Session 1 Overview:

  • Methods of antigen detection
  • Defining your imaging methods
  • Establishing your detection priorities
  • Reagent selection

Welcome! If you're not already enrolled in this course, sign up here to get the rest of the sessions emailed to you for free.

In this section we’re going to select reagents for priorities you have in your laboratory, which can include:

  • Sensitivity
  • Simplicity
  • Flexibility
  • Rapid results
  • Cost
  • Reproducibility

Welcome! If you're not already enrolled in this course, sign up here to get the rest of the sessions emailed to you for free.

In this section we’re going to select ancillary reagents which help to improve staining and complete your workflow. We will discuss:

  • Antigen unmasking, antigen retrieval, and primary-antibody diluent
  • Counterstaining
  • Mounting Media
  • Blocking Reagents

 

Welcome! If you're not already enrolled in this course, sign up here to get the rest of the sessions emailed to you for free.

In this session we’re going to cover optimizing your protocol. This includes:

  • 1:22 Optimization Step 1: Primary antibody
  • 1:59 Optimization Step 2: Detection system
  • 2:30 Optimization Step 3: Background signal

Welcome! If you're not already enrolled in this course, sign up here to get all the sessions emailed to you for free.

In this session we’re going to cover advanced detection protocols. Inside we’ll explore:

  • 1:10 Multiplexing - detecting multiple antigens simultaneously
  • 5:34 Species-on-species detection