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Welcome to Session 1 of Considerations For Designing Immunohistochemical Procedures. In this session we’re going to cover the essentials of immunohistochemistry.

Session 1 Overview:

• Methods of antigen detection
• Defining your imaging methods
• Establishing your detection priorities
• Reagent selection

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In this section we’re going to select reagents for priorities you have in your laboratory, which can include:

• Sensitivity
• Simplicity
• Flexibility
• Rapid results
• Cost
• Reproducibility

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In this section we’re going to select ancillary reagents which help to improve staining and complete your workflow. We will discuss:

• Antigen unmasking, antigen retrieval, and primary-antibody diluent
• Counterstaining
• Mounting Media
• Blocking Reagents

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In this session we’re going to cover optimizing your protocol. This includes:

• 1:22 Optimization Step 1: Primary antibody
• 1:59 Optimization Step 2: Detection system
• 2:30 Optimization Step 3: Background signal

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In this session we’re going to cover advanced detection protocols. Inside we’ll explore:

• 1:10 Multiplexing - detecting multiple antigens simultaneously
• 5:34 Species-on-species detection