Dramatically Reduce Autofluorescence
Reveal true immunofluorescence - even in the most challenging tissues
Serial sections of human kidney (FFPE) counterstained with DAPI (blue nuclei). Untreated section (left) with high autofluorescence in the green channel. TrueVIEW treated section shows elimination of tissue autoflorescence in green channel while retaining specific DAPI staining.
Decrease Autofluorescence with
Vector® TrueVIEWTM Autofluorescence Quenching Kit
Specific reduction of autofluorescence from non-lipofuscin sources
Easy-to-use, one-step method, quick 5 min incubation
Compatible with a wide selection of fluorophores
Compatible with standard epifluorescence and confocal laser microscopes
Autofluorescence is the inherent unwanted fluorescence in the tissue that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background.
The novel, patent-pending Vector® TrueVIEWTM Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays.
Interested in learning more?
Download the award winning poster describing TrueVIEWTM Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.
"The TrueVIEWTMdefinitely decreased autofluorescence more than the competition while seeming to have no effect on GFP signal."
Why researchers are choosing
Vector® TrueVIEWTM for their workflow
Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the Vector® TrueVIEWTM Quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin.
It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen and pancreas.
Competitor and other autofluorescence treatments
Results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas avisualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.
Download the award-winning poster to learn more!
Download the poster describing the TrueVIEWTM Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.
Download the Poster
Fill out the information below to view the TrueVIEWTM Autofluorescence Quenching Kit poster: